![]() H., Miyoshi, G., Hjerling-Leffler, J., Karayannis, T. Hippo pathway deletion in adult resting cardiac fibroblasts initiates a cell state transition with spontaneous and self-sustaining fibrosis. The encyclopedia of DNA elements (ENCODE): data portal update. Lines of murine oligodendroglial precursor cells immortalized by an activated neu tyrosine kinase show distinct degrees of interaction with axons in vitro and in vivo. Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system. Altered human oligodendrocyte heterogeneity in multiple sclerosis. Transcriptional convergence of oligodendrocyte lineage progenitors during development. Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis. High-throughput single-cell ChIP–seq identifies heterogeneity of chromatin states in breast cancer. Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT–seq). Single-cell chromatin immunocleavage sequencing (scChIC–seq) to profile histone modification. CoBATCH for high-throughput single-cell epigenomic profiling. Profiling chromatin states using single-cell itChIP–seq. Single-cell ChIP–seq reveals cell subpopulations defined by chromatin state. CUT&Tag for efficient epigenomic profiling of small samples and single cells. An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. Single-cell DNA-methylation analysis reveals epigenetic chimerism in preimplantation embryos. ![]() ![]() Single-cell chromatin accessibility reveals principles of regulatory variation. Molecular architecture of the mouse nervous system. Our results indicate that analysis of histone modifications and transcription factor occupancy at single-cell resolution provides unique insights into epigenomic landscapes in the central nervous system. We also used scCUT&Tag to investigate the single-cell chromatin occupancy of transcription factor OLIG2 and the cohesin complex component RAD21. These scCUT&Tag profiles were sufficient to determine cell identity and deconvolute regulatory principles such as promoter bivalency, spreading of H3K4me3 and promoter–enhancer connectivity. We apply single-cell CUT&Tag (scCUT&Tag) to tens of thousands of cells of the mouse central nervous system and probe histone modifications characteristic of active promoters, enhancers and gene bodies (H3K4me3, H3K27ac and H3K36me3) and inactive regions (H3K27me3). Here, we combine the CUT&Tag technology, developed to measure bulk histone modifications, with droplet-based single-cell library preparation to produce high-quality single-cell data on chromatin modifications. In contrast to single-cell approaches for measuring gene expression and DNA accessibility, single-cell methods for analyzing histone modifications are limited by low sensitivity and throughput. ![]()
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